• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Glu24-Asn446
Catalog #LIL-HM4A4
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human LILRA4/CD85g Protein (LIL-HM4A4) Summary

Product Description

 Recombinant Human LILRA4/CD85g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Asn446.

Product Background

 LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain.LILRA4 function coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as negative regulator of TLR7 and TLR9 signaling cascades.

Non-biotinylated Human LILRA4/CD85g Protein (LIL-HM4A4) Specifications/Details

Protein
 LILRA4/CD85g
Synonyms
 CD85g; ILT7; ILT-7; ILT7MGC129598; LILRA4; MGC129597
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu24-Asn446
Molecular Weight
 The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LILRA4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human LILRA4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human LILRA4 is greater than 95% as determined by SEC-HPLC. The purity of Human LILRA4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...