• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LOX1, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-LOX1 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
  • Amino Acid Range: Ser61-Gln273
Catalog #LOX-HM101
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human LOX1 Protein (LOX-HM101) Summary

Product Description

 Recombinant Human LOX1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser61-Gln273.

Product Background

 LOX-1 is a transmembrane glycoprotein that binds to and internalizes ox-LDL.LOX-1 gene deletion in mice and anti-LOX-1 therapy has been shown to decrease inflammation, oxidative stress and atherosclerosis. LOX-1 deletion also results in damage from ischemia, making LOX-1 a promising target of therapy for atherosclerosis and related disorders. In this article we focus on the different mechanisms for regulation, signaling and the various effects of LOX-1 in contributing to atherosclerosis.

Human LOX1 Protein (LOX-HM101) Specifications/Details

Protein
 LOX1
Synonyms
 Ox-LDL receptor 1; LOX-1; CLEC8A; LOX1; OLR1; LOXIN; SR-E1; SCARE1; SLOX1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser61-Gln273
Molecular Weight
 The protein has a predicted MW of 25.4 kDa. Due to glycosylation, the protein migrates to 30-36 kDa under reduced condition and 60-70 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human LOX1, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-LOX1 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LOX1, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-LOX1 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. Immobilized Human LOX1, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-LOX1 Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.
Human LOX1 on Bis-Tris PAGE under reduced condition and Non reduced (N) condition. The purity is greater than 95%. Human LOX1 on Bis-Tris PAGE under reduced condition and Non reduced (N) condition. The purity is greater than 95%.
The purity of Human LOX1 is greater than 95% as determined by SEC-HPLC. The purity of Human LOX1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...