• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gly20-Cys709
Catalog #MFI-HM128
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

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Human CD228/MFI2 Protein (MFI-HM128) Summary

Product Description

 Recombinant Human CD228/MFI2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys709.

Product Background

 Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.

Human CD228/MFI2 Protein (MFI-HM128) Specifications/Details

Protein
 CD228/MFI2
Synonyms
 Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly20-Cys709
Molecular Weight
 The protein has a predicted MW of 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). Immobilized Human CD228, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-CD228 Ab., hFc-Avi Tag on ProA-Biosensor can bind Human CD228, His Tag with an affinity constant of 1.54 nM as determined in BLI assay . Loaded Anti-CD228 Ab., hFc-Avi Tag on ProA-Biosensor can bind Human CD228, His Tag with an affinity constant of 1.54 nM as determined in BLI assay .
The purity of Human CD228 is greater than 95% as determined by SEC-HPLC. The purity of Human CD228 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...