• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide
Catalog #MHC-HM443T
Price
Regular price $825.00
Regular price Sale price $825.00
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Size
Avantor VWR Fisher Scientific

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Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443T) Summary

Product Description

 Recombinant Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide.

Product Background

 PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Specifications

Protein
 HLA-A*02:01&B2M&PRAME (SLLQHLIGL)
Synonyms
 PRAME; OIP-4; OIP4; MAPE;
MHC Form
 Tetramer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide
Molecular Weight
 The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA. Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 9.9 ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
Loaded HLA-A*02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay . Loaded HLA-A*02:01&B2M&PRAME TCR, hFc Tag on ProA-Biosensor can bind Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer with an affinity constant of 48.80 pM as determined in BLI assay .
The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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