• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Pro2-Ala115
Catalog #MIF-HM101
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human MIF Protein (MIF-HM101) Summary

Product Description

 Recombinant Human MIF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro2-Ala115.

Product Background

 Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine with chemokine-like functions that increasingly is being studied in different aspects of cardiovascular disease. MIF was first identified as a proinflammatory and pro-survival mediator within the immune system, and a second structurally related MIF family member, D-dopachrome tautomerase (a.k.a. MIF-2), was reported recently.

Human MIF Protein (MIF-HM101) Specifications/Details

Protein
 MIF
Synonyms
 GIF; L-dopachrome isomerase; GLIF; MMIF; EC 5.3.2.1; EC 5.3.3.12;EC5.3.2.1; EC5.3.3.12
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro2-Ala115
Molecular Weight
 The protein has a predicted MW of 13.44 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human MIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...