Human MARCO Protein (MRO-HM102)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Ser405-Val520

Catalog #MRO-HM102

Price

$346⁵⁰

Size

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Human MARCO Protein (MRO-HM102) Summary

Product Description

Recombinant Human MARCO Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser405-Val520.

Product Background

Macrophage receptor with collagenous structure (MARCO) is the predominant scavenger receptor for recognition and binding of silica particles by alveolar macrophages (AM). MARCO contributes to normal cholesterol uptake in macrophages; therefore, in the absence of MARCO, macrophages are more susceptible to a greater inflammatory response by particulates known to cause NLRP3 inflammasome activation and the effect is due to increased LMP.

Human MARCO Protein (MRO-HM102) Specifications/Details

Protein	MARCO
Synonyms	MARCO; SCARA2; AI323439; Ly112
Accession	Q9UEW3-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser405-Val520
Molecular Weight	The protein has a predicted MW of 13.8 kDa. Due to glycosylation, the protein migrates to 14-20 kDa based on Bis-Tris PAGE result.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human MARCO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human MARCO is greater than 95% as determined by SEC-HPLC.