• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gly58-Asp400
Catalog #NEC-HM403
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human Nectin-3/CD113 Protein (NEC-HM403) Summary

Product Description

 Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400.

Product Background

 The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.

Non-biotinylated Human Nectin-3/CD113 Protein (NEC-HM403) Specifications/Details

Protein
 Nectin-3/CD113
Synonyms
 CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly58-Asp400
Molecular Weight
 The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200). Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC. The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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