• Express System: HEK293
  • Product Tag: C-His, C-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
  • Amino Acid Range: Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)
Catalog #NKC-CM194
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus NKG2A&CD94 Protein (NKC-CM194) Summary

NKC-CM194 Product Description
 Recombinant Cynomolgus NKG2A&CD94 Protein is expressed from HEK293 with His tag at the C-Terminus of NKG2A and Flag tag at the C-Terminus of CD94.It contains Pro94-Leu233(NKG2A) and Lys32-Ile179(CD94).

Product Background

 The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Protein
 NKG2A&CD94
Synonyms
 CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)
Molecular Weight
 The protein has a predicted MW of 17.01 kDa(NKG2A)&18.27 kDa(CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Product Tag
 C-His, C-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized Cynomolgus NKG2A&CD94, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Cynomolgus NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus NKG2A&CD94 is greater than 90% as determined by SEC-HPLC. The purity of Cynomolgus NKG2A&CD94 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...