• Express System: HEK293
  • Product Tag: N-His-Avi, N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, His Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Catalog #NKC-HM495
Price
Regular price $346.50
Regular price Sale price $346.50
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human NKG2A&CD94 Protein (NKC-HM495) Summary

Product Description

 Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2A and Flag tag at the N-Terminus of CD94.It contains Arg100-Leu233(NKG2A) and Ser34-Ile179(CD94).

Product Background

 The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Non-biotinylated Human NKG2A&CD94 Protein (NKC-HM495) Specifications/Details

Protein
 NKG2A&CD94
Synonyms
 CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight
 The protein has a predicted MW of 17.9 kDa (CD94) and 18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NKG2A&CD94, His Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi, N-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human NKG2A&CD94, His Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). Immobilized Human NKG2A&CD94, His Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag : Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml. Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag : Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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