• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Arg100-Ala219
Catalog #NKG-HM210
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human NKG2A/CD159a Protein (NKG-HM210) Summary

Product Description

 Recombinant Human NKG2A/CD159a Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Arg100-Ala219.

Product Background

 NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.

Human NKG2A/CD159a Protein (NKG-HM210) Specifications/Details

Protein
 NKG2A/CD159a
Synonyms
 NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg100-Ala219
Molecular Weight
 The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test).
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test).
Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKG2A is greater than 95% as determined by SEC-HPLC. The purity of Human NKG2A is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...