• Express System: HEK293
  • Product Tag: N-hFc (IgG1)-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Phe78-Val216
Catalog #NKG-HM22D
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human NKG2D/CD314 Protein (NKG-HM22D) Summary

Product Description

 Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc (IgG1) tag and Flag tag at the N-Terminus.It contains Phe78-Val216.

Product Background

 NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Human NKG2D/CD314 Protein (NKG-HM22D) Specifications/Details

Protein
 NKG2D/CD314
Synonyms
 CD314; D12S2489E; KLR; NKG2-D; NKG2D
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe78-Val216
Molecular Weight
 The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
Product Tag
 N-hFc (IgG1)-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
Immobilized Human MICB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA Immobilized Human MICB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA
Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml. Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
Loaded Human NKG2D, hFc Tag on ProA-Biosensor can bind Cynomolgus ULBP-2, His Tag (Cat. ULB-CM102) with an affinity constant of 30.00 nM as determined in BLI assay . Loaded Human NKG2D, hFc Tag on ProA-Biosensor can bind Cynomolgus ULBP-2, His Tag (Cat. ULB-CM102) with an affinity constant of 30.00 nM as determined in BLI assay .
The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC. The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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