• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
  • Amino Acid Range: Arg100-Leu233
Catalog #NKG-HM410B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human NKG2A/CD159a Protein (NKG-HM410B) Summary

Product Description

 Recombinant Biotinylated Human NKG2A/CD159a Protein is expressed from HEK293 with His tag and Avi tag at N-Terminus.It contains Arg100-Leu233.

Product Background

 NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.

Biotinylated Human NKG2A/CD159a Protein (NKG-HM410B) Specifications/Details

Protein
 NKG2A/CD159a
Synonyms
 NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Arg100-Leu233
Molecular Weight
 The protein has a predicted MW of 18.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Product Tag
 N-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. Immobilized Biotinylated Human NKG2A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
Biotinylated Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...