• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala1378-Ser1640
Catalog #NOT-HM103
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human Notch 3 Protein (NOT-HM103) Summary

Product Description

 Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.

Product Background

 Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Human Notch 3 Protein (NOT-HM103) Specifications/Details

Protein
 Notch 3
Synonyms
 CADASIL; Notch homolog 3; Notch-3; NOTCH3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala1378-Ser1640
Molecular Weight
 The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC. The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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