• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu26-Gln530
Catalog #NOT-HM402B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Notch 2 Protein (NOT-HM402B) Summary

Product Description

 Recombinant Biotinylated Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Gln530.

Product Background

 Human Notch-2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch-2 is lethal, showing that not all functions overlap.Notch-2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.

Biotinylated Human Notch 2 Protein (NOT-HM402B) Specifications/Details

Protein
 Notch 2
Synonyms
 AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu26-Gln530
Molecular Weight
 The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human Notch 2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human Notch 2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human Notch 2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Notch 2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...