• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 1.26ug/ml determined by ELISA.
  • Amino Acid Range: Phe22-Lys644
Catalog #NRP-HM101
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human Neuropilin-1 Protein (NRP-HM101) Summary

Product Description

 Recombinant Human Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Lys644.

Product Background

 Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta.

Human Neuropilin-1 Protein (NRP-HM101) Specifications/Details

Protein
 Neuropilin-1
Synonyms
 BDCA4; NP1; Neuropilin-1; NRP1; CD304; VEGF165R; Neuropilin 1; NRP; Transmembrane Receptor; CD304 Antigen
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe22-Lys644
Molecular Weight
 The protein has a predicted MW of 70.97 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 1.26ug/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 1.26ug/ml determined by ELISA. Immobilized Human VEGF165, No Tag (Cat. VEG-HM065) at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 1.26ug/ml determined by ELISA.
Human Neuropilin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Neuropilin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Neuropilin-1 is greater than 90% as determined by SEC-HPLC. The purity of Human Neuropilin-1 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...