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Non-biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440) Summary
Product Description |
Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216. |
Product Background |
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440) Specifications/Details
Protein |
OX40/TNFRSF4/CD134 |
Synonyms |
CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu29-Ala216 |
Molecular Weight |
The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). |
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Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human OX40 is greater than 95% as determined by SEC HPLC. |
Other Available Proteins:
Finding available proteins...
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