• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Leu29-Ala216
Catalog #OX4-HM440
Price
Regular price $396.00
Regular price Sale price $396.00
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Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440) Summary

Product Description

 Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216.

Product Background

 Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Non-biotinylated Human OX40/TNFRSF4/CD134 Protein (OX4-HM440) Specifications/Details

Protein
 OX40/TNFRSF4/CD134
Synonyms
 CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16;ACT135; Ly70
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu29-Ala216
Molecular Weight
 The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human OX40 is greater than 95% as determined by SEC HPLC. The purity of Human OX40 is greater than 95% as determined by SEC HPLC.
Other Available Proteins:
Finding available proteins...