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Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (PDL-CM210) Summary
Product Description |
Recombinant Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe19-Arg238. |
Product Background |
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins, ADC targets |
Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (PDL-CM210) Specifications/Details
Protein |
PD-L1/B7-H1 |
Synonyms |
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Phe19-Arg238 |
Molecular Weight |
The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus PD-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque PD-L1, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Cynomolgus PD-1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque PD-L1, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. |
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Immobilized Cynomolgus/Rhesus macaque PD-L1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD-L1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. |
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Cynomolgus/Rhesus macaque PD-L1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Cynomolgus/Rhesus macaque PD-L1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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