• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Val19-Pro158
Catalog #PGF-MM101
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Mouse PGF Protein (PGF-MM101) Summary

Product Description

 Recombinant Mouse PGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro158.

Product Background

 Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.

Mouse PGF Protein (PGF-MM101) Specifications/Details

Protein
 PGF
Synonyms
 PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2;SHGC10760; PlGF2
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Val19-Pro158
Molecular Weight
 The protein has a predicted MW of 16.99 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test). Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test).
Mouse PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...