• Express System: HEK293
  • Product Tag: C-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test). The affinity constant of 2.22 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ser35-Asp165
Catalog #PVR-MM501B
Price
Regular price $57750
Regular price Sale price $57750
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Size
Avantor VWR Fisher Scientific

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Biotinylated Mouse PVRIG Protein (PVR-MM501B) Summary

Product Description

 Recombinant Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Ser35-Asp165.

Product Background

 Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.

Biotinylated Mouse PVRIG Protein (PVR-MM501B) Specifications/Details

Protein
 PVRIG
Synonyms
 C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1;RalGDS/AF6
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Ser35-Asp165
Molecular Weight
 The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test). The affinity constant of 2.22 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 uM as determined in SPR assay (Biacore T200). Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test). Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test).
Biotinylated Mouse PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Mouse PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...