• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gly24-Tyr197
Catalog #RNF-HM234
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human RNF43 Protein (RNF-HM234) Summary

Product Description

 Recombinant Human RNF43 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly24-Tyr197.

Product Background

 RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

Human RNF43 Protein (RNF-HM234) Specifications/Details

Protein
 RNF43
Synonyms
 E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly24-Tyr197
Molecular Weight
 The protein has a predicted MW of 45.8 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test). Immobilized Human R-Spondin 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA (QC Test).
Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA. Immobilized Human R spondin 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4ug/ml determined by ELISA.
Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay . Loaded Human R spondin 1, His Tag on Anti-His-Biosensor can bind Human RNF43, hFc Tag with an affinity constant of 18.50 nM as determined in BLI assay .
The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC. The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...