• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala27-Glu469
Catalog #ROB-HM104
Price
Regular price $29700
Regular price Sale price $29700
/
Size
Avantor VWR Fisher Scientific

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Human ROBO4 Protein (ROB-HM104) Summary

Product Description

 Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.

Product Background

 Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.

Human ROBO4 Protein (ROB-HM104) Specifications/Details

Protein
 ROBO4
Synonyms
 ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Glu469
Molecular Weight
 The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
Human ROBO4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ROBO4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC. The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...