• Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Amino Acid Range: Glu1541-Ile1854
Catalog #SAR-VE1PL
Price
Regular price $16500
Regular price Sale price $16500
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated SARS PLpro/papain-like protease Protein (SAR-VE1PL) Summary

Product Description

 Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus.It contains Glu1541-Ile1854.

Product Background

 The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.

Non-biotinylated SARS PLpro/papain-like protease Protein (SAR-VE1PL) Specifications/Details

Protein
 PLpro/papain-like protease
Synonyms
 Replicase polyprotein 1a; pp1a; nsp1; PL2-PRO; PL-PRO; Papain-like Protease; Plpro;PL2PRO
Accession
Species
 SARS
Biotinylated
 no
Amino Acid Range
 Glu1541-Ile1854
Molecular Weight
 The protein has a predicted MW of 38.6 kDa same as Bis-Tris PAGE result.
Product Tag
 N-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 7.8).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

SARS-CoV-2 PLpro on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. SARS-CoV-2 PLpro on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS PLpro is greater than 85% as determined by SEC-HPLC. The purity of SARS PLpro is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...