• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Gln45-Ala646
Catalog #SEM-MM17A
Price
Regular price $239.80
Regular price Sale price $239.80
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Size
Avantor VWR Fisher Scientific

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Mouse SEMA7A Protein (SEM-MM17A) Summary

Product Description

 Recombinant Mouse SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ala646.

Product Background

 Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.

Mouse SEMA7A Protein (SEM-MM17A) Specifications/Details

Protein
 SEMA7A
Synonyms
 Semaphorin-7A; Semaphorin-K1; Semaphorin-L; Sema L; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH; MGC126692; Sema7A; SEMAK1; SEMAL
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gln45-Ala646
Molecular Weight
 The protein has a predicted MW of 69.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse SEMA7A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse SEMA7A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SEMA7A is greater than 90% as determined by SEC-HPLC. The purity of Mouse SEMA7A is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...