• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Glu17-Thr434
Catalog #SIG-HM205
Price
Regular price $23100
Regular price Sale price $23100
/
Size
Avantor VWR Fisher Scientific

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Human Siglec-5/CD170 Protein (SIG-HM205) Summary

Product Description

 Recombinant Human Siglec-5/CD170 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu17-Thr434.

Product Background

 Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.

Human Siglec-5/CD170 Protein (SIG-HM205) Specifications/Details

Protein
 Siglec-5/CD170
Synonyms
 CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu17-Thr434
Molecular Weight
 The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Siglec-5 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Siglec-5 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC. The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...