Non-biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411)

Immobilized Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).

Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).
Amino Acid Range: Met17-Thr546 (R119A)

Catalog #SIG-HM411

Price

$346⁵⁰

Size

Order by 4pm ET for delivery on:

Non-biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411) Summary

Product Description

Recombinant Human Siglec-10 (R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Product Background

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Non-biotinylated Human Siglec-10 (R119A) Protein (SIG-HM411) Specifications/Details

Protein	Siglec-10
Synonyms	SLG2; SIGLEC10; MGC126774; PRO940
Accession	Q96LC7-1
Species	Human
Biotinylated	no
Amino Acid Range	Met17-Thr546 (R119A)
Molecular Weight	The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris,150mM NaCl, 200mM Arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).
	Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.