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Non-biotinylated Human Siglec-9 Protein (SIG-HM419) Summary
Product Description |
Recombinant human Siglec-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Gly348. |
Product Background |
Siglec-9 is a sialic-acid-binding lectin expressed predominantly on myeloid cells. Aberrant glycosylation occurs in essentially all types of cancers and results in increased sialylation. Thus, when the mucin MUC1 is expressed on cancer cells, it is decorated by multiple short, sialylated O-linked glycans (MUC1-ST). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human Siglec-9 Protein (SIG-HM419) Specifications/Details
Protein |
Siglec-9 |
Synonyms |
CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln18-Gly348 |
Molecular Weight |
The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 25mM MES (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human Siglec-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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