• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Ser23-Met226
Catalog #SMF-HM407B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human SLAMF7/CRACC/CD319 Protein (SMF-HM407B) Summary

Product Description

 Recombinant Biotinylated Human SLAMF7/CRACC/CD319 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser23-Met226

Product Background

 CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 65-75 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family.Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response.

Biotinylated Human SLAMF7/CRACC/CD319 Protein (SMF-HM407B) Specifications/Details

Protein
 SLAMF7/CRACC/CD319
Synonyms
 CD2 subset 1; CRACC; Novel Ly9; CD319; SLAMF7; CS1; 19A; FOAP-12;FOAP12
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ser23-Met226
Molecular Weight
 The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Biotinylated Human SLAMF7, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Biotinylated Human SLAMF7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human SLAMF7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SLAMF7 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human SLAMF7 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...