• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA.
  • Amino Acid Range: Glu29-Pro360
Catalog #SRP-HM40G
Price
Regular price $247.50
Regular price Sale price $247.50
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40G) Summary

Product Description

 Recombinant Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.

Product Background

 Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

Non-biotinylated Human SIRP gamma/CD172g Protein (SRP-HM40G) Specifications/Details

Protein
 SIRP gamma/CD172g
Synonyms
 CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2; SIRPγ; SIRP γ;SIRP b 2; SIRP b2; SIRP g
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu29-Pro360
Molecular Weight
 The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA. Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA.
Human SIRP gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human SIRP gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human SIRP gamma is greater than 90% as determined by SEC-HPLC. The purity of Human SIRP gamma is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...