• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)
Catalog #SRP-HM4V8B
Price
Regular price $577.50
Regular price Sale price $577.50
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B) Summary

Product Description

 Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A).

Product Background

 Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Biotinylated Human SIRP alpha V8 Protein (SRP-HM4V8B) Specifications/Details

Protein
 SIRP alpha V8
Synonyms
 CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA; SIRPα
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)
Molecular Weight
 The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Biotinylated Human SIRP alpha V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human SIRP alpha V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human SIRP alpha V8 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human SIRP alpha V8 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...