• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 0.75 nM.
  • Amino Acid Range: Asp29-Lys282
Catalog #TFP-HM401
Price
Regular price $31350
Regular price Sale price $31350
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human TFPI Protein (TFP-HM401) Summary

Product Description

 Recombinant Human TFPI Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp29-Lys282.

Product Background

 Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development.

Non-biotinylated Human TFPI Protein (TFP-HM401) Specifications/Details

Protein
 TFPI
Synonyms
 LACI; TFPI1; EPI; TFI; convertin; TFPI
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp29-Lys282
Molecular Weight
 The protein has a predicted MW of 32.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 0.75 nM.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TFPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TFPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TFPI is greater than 95% as determined by SEC-HPLC. The purity of Human TFPI is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...