• Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala303-Ser414
Catalog #TG2-HM00MB
Price
Regular price $66000
Regular price Sale price $66000
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Mature TGF beta 2 Protein (TG2-HM00MB) Summary

Product Description

 Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala303-Ser414.

Product Background

 Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.

Biotinylated Human Mature TGF beta 2 Protein (TG2-HM00MB) Specifications/Details

Protein
 Mature TGF beta 2
Synonyms
 TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2;TGF beta R2; TbR-II; T beta R-II; TGF-b 2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala303-Ser414
Molecular Weight
 The protein has a predicted MW of 14.53 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
Product Tag
 C-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Other Available Proteins:
Finding available proteins...