• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met22-Pro141
Catalog #TIG-HM210
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Human TIGIT Protein (TIG-HM210) Summary

Product Description

 Recombinant Human TIGIT Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Met22-Pro141.

Product Background

 TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Human TIGIT Protein (TIG-HM210) Specifications/Details

Protein
 TIGIT
Synonyms
 TIGIT; VSIG9; VSTM3; WUCAM
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met22-Pro141
Molecular Weight
 The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test). Immobilized Human TIGIT, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. Immobilized Biotinylated Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human TIGIT, hFc Tag on Anti-Human lgG Fc Gen II (HFc II) -Biosensor can bind Biotinylated Human CD155, His-Avi Tag (Cat. CD1-HM455B) with an affinity constant of 0.72 nM as determined in BLI assay. Loaded Human TIGIT, hFc Tag on Anti-Human lgG Fc Gen II (HFc II) -Biosensor can bind Biotinylated Human CD155, His-Avi Tag (Cat. CD1-HM455B) with an affinity constant of 0.72 nM as determined in BLI assay.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC. The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...