• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus TREM2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA.
  • Amino Acid Range: His19-Ser174
Catalog #TRM-CM401
Price
Regular price $264.00
Regular price Sale price $264.00
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Size
Avantor VWR Fisher Scientific

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Cynomolgus TREM2 Protein (TRM-CM401) Summary

Product Description

 Recombinant Cynomolgus TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His19-Ser174.

Product Background

 TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2- consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

Cynomolgus TREM2 Protein (TRM-CM401) Specifications/Details

Protein
 TREM2
Synonyms
 PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 His19-Ser174
Molecular Weight
 The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 33-42 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus TREM2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus TREM2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA. Immobilized Cynomolgus TREM2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA.
Cynomolgus TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
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