Human TYRO3 Protein (TYR-HM103)

Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).

Human TYRO3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TYRO3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala41-Trp429

Catalog #TYR-HM103

Price

$165⁰⁰

Size

Order by 4pm ET for delivery on:

Human TYRO3 Protein (TYR-HM103) Summary

Product Description

Recombinant Human TYRO3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala41-Trp429.

Product Background

The TAM receptors (Tyro3, Axl and MerTK) are promising therapeutic targets on tumor-associated macrophages. The TAM receptors are a family of receptor tyrosine kinases with shared ligands Gas6 and Protein S that skew macrophage polarization towards a pro-tumor M2-like phenotype.In macrophages, the TAM receptors also promote apoptotic cell clearance, a tumor-promoting process called efferocytosis. The TAM receptors bind the "eat-me" signal phosphatidylserine on apoptotic cell membranes using Gas6 and Protein S as bridging ligands.

Human TYRO3 Protein (TYR-HM103) Specifications/Details

Protein	TYRO3
Synonyms	BYK; DTK; RSE; SKY; TIF; TYRO3; Brt; Rse
Accession	Q06418
Species	Human
Biotinylated	no
Amino Acid Range	Ala41-Trp429
Molecular Weight	The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 uM as determined in SPR assay (Biacore T200).
	Human TYRO3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TYRO3 is greater than 95% as determined by SEC-HPLC.