• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM465
Price
Regular price $330.00
Regular price Sale price $330.00
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human VEGF165 Protein (VEG-HM465) Summary

Product Description

 Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.

Product Background

 Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Non-biotinylated Human VEGF165 Protein (VEG-HM465) Specifications/Details

Protein
 VEGF165
Synonyms
 VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165;VEGF165;Vascular endothelial growth factor-165; Vascular endothelial growth factor 165
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Arg191
Molecular Weight
 The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA. Immobilized Human VEGF165 at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 4.1 ng/ml determined by ELISA.
Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody2, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. Immobilized Human VEGF165, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody2, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 90%. Human VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 90%.
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay. Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R1, hFc Tag with an affinity constant of 0.94 nM as determined in BLI assay.
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay. Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Human VEGF R2, hFc Tag with an affinity constant of 2.34 nM as determined in BLI assay.
Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay. Loaded Human VEGF165, His-Avi Tag on Anti-His-Biosensor can bind Anti-VEGF165 Antibody, hFc-Avi Tag with an affinity constant of 31.50 pM as determined in BLI assay.
The purity of Human VEGF165 is greater than 90% as determined by SEC-HPLC. The purity of Human VEGF165 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...