• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
  • Amino Acid Range: Thr103-Arg227
Catalog #VEG-HM4F1B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human VEGF-C/Flt4-L Protein (VEG-HM4F1B) Summary

Product Description

 Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227.

Product Background

 the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.

Biotinylated Human VEGF-C/Flt4-L Protein (VEG-HM4F1B) Specifications/Details

Protein
 VEGF-C/Flt4-L
Synonyms
 VEGFC; VEGFc; Flt4-L; VRP; VEGF-C;Flt4L
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Thr103-Arg227
Molecular Weight
 The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in 50mM MES (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Biotinylated Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%. Biotinylated Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...