• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-CM1R2
Price
Regular price $264.00
Regular price Sale price $264.00
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Size
Avantor VWR Fisher Scientific

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Rhesus macaque VEGF R2/KDR Protein (VGF-CM1R2) Summary

Product Description

 Recombinant Rhesus macaque VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Glu764.

Product Background

 The vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/flk-1) functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. Transcriptional regulation of the VEGFR-2 is complex and may involve multiple putative upstream regulatory elements including E boxes.

Rhesus macaque VEGF R2/KDR Protein (VGF-CM1R2) Specifications/Details

Protein
 VEGF R2/KDR
Synonyms
 CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73;VEGFR21
Accession
Species
 Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Ala20-Glu764
Molecular Weight
 The protein has a predicted MW of 84.37 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rhesus macaque VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rhesus macaque VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). Immobilized Rhesus macaque VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Rhesus macaque VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rhesus macaque VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque VEGF R2 is greater than 95% as determined by SEC-HPLC. The purity of Rhesus macaque VEGF R2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...