Regular price
$577.50
Order by 4pm ET for delivery on:
Biotinylated Human VEGF R1/FLT-1 Protein (VGF-HM4R1B) Summary
Product Description |
Recombinant Biotinylated Human VEGF R1/FLT-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser27-Asn756. |
Product Background |
VEGFR1 (vascular endothelial growth factor receptor 1), also called Flt-1 (Fms-like tyrosine kinase), is a 180 kDa type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTKs).yrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Biotinylated Human VEGF R1/FLT-1 Protein (VGF-HM4R1B) Specifications/Details
Protein |
VEGF R1/FLT-1 |
Synonyms |
FLT; FLT1; Flt-1; FRT; VEGF R1; VEGFR1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser27-Asn756 |
Molecular Weight |
The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test). |
|
|
Immobilized Human VEGF121, His Tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 48.8ng/ml determined by ELISA. |
|
|
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 48ng/ml determined by ELISA. |
|
|
Biotinylated Human VEGF R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
