• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ser31-Tyr538
Catalog #VZV-VM10E
Price
Regular price $264.00
Regular price Sale price $264.00
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein (VZV-VM10E) Summary

Product Description

 Recombinant Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser31-Tyr538.

Product Background

 VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen.

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein (VZV-VM10E) Specifications/Details

Protein
 Envelope glycoprotein E
Synonyms
 Envelope glycoprotein E; gE; ORF68;
Accession
Species
 Varicella-zoster virus (strain Oka vaccine)
Biotinylated
 no
Amino Acid Range
 Ser31-Tyr538
Molecular Weight
 The protein has a predicted MW of 58.39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E is greater than 95% as determined by SEC-HPLC. The purity of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...