• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human YKL-40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Tyr22-Thr383
Catalog #YKL-HM140
Price
Regular price $23100
Regular price Sale price $23100
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Size
Avantor VWR Fisher Scientific

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Human YKL-40/CHI3L1 Protein (YKL-HM140) Summary

Product Description

 Recombinant Human YKL-40/CHI3L1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Thr383.

Product Background

 YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role is not completely understood but YKL-40 is elevated in the brain and cerebrospinal fluid (CSF) in several neurological and neurodegenerative diseases associated with inflammatory processes. Yet the precise characterization of YKL-40 in dementia cases is missing. YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases. Therefore, YKL-40 quantification might have a potential for application in the evaluation of therapeutic intervention in dementias with a neuroinflammatory component.

Human YKL-40/CHI3L1 Protein (YKL-HM140) Specifications/Details

Protein
 YKL-40/CHI3L1
Synonyms
 BRP39; CGP-39; GP-39; Chil1; ASRT7; FLJ38139; HC-gp39; YKL-40; YKL40; YYL-40; AW208766; CHI3L1; Chitinase 3 like 1;CGP39; GP39; YYL40
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr22-Thr383
Molecular Weight
 The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human YKL-40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human YKL-40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC Test). Immobilized Human YKL-40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA (QC Test).
Human YKL-40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human YKL-40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human YKL-40 is greater than 95% as determined by SEC-HPLC. The purity of Human YKL-40 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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