Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein (MHC-HM448B)
Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein (MHC-HM448B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide
Catalog #MHC-HM448B
Regular price $310.00
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Product Description

Recombinant Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide.

Product Background

HLA-B*15:01 is strongly associated with asymptomatic infection with SARS-CoV-2 and is likely to be involved in the mechanism underlying early viral clearance. T cells from pre-pandemic individuals carrying HLA-B*15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer

Synonyms

MHC; SARS-CoV-2 epitope;

MHC Form

Monomer

Allele

HLA-B*15:01

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide

Molecular Weight

The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC.

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