Biotinylated Canine CD20/MS4A1 Nanodisc (CD2-DM12NB)

SKU: CD2-DM12NB-100UG

Biotinylated Canine CD20/MS4A1 Nanodisc (CD2-DM12NB)

SKU: CD2-DM12NB-100UG
Biotinylated Canine CD20/MS4A1 Nanodisc (CD2-DM12NB)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Canine CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. The affinity constant of 1.01 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met1-Pro297
Catalog #CD2-DM12NB
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Size
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Biotinylated Canine CD20/MS4A1 Nanodisc (CD2-DM12NB) Summary

Product Description

 Recombinant Biotinylated Canine CD20/MS4A1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Pro297.

Product Background

 B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

Product Category

 Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc, ADC targets

Biotinylated Canine CD20/MS4A1 Nanodisc (CD2-DM12NB) Specifications/Details

Protein
 CD20/MS4A1
Synonyms
 MS4A1; CD20; MS4A-1; B1; Bp35; CD20 receptor; CD20S7; CVID5; LEU-16; Ly-44; MS4A2; S7
Accession
Species
 Canine
Biotinylated
 no
Amino Acid Range
 Met1-Pro297
Molecular Weight
 The protein has a predicted MW of 46.01 kDa.
Activity
 Immobilized Biotinylated Canine CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. The affinity constant of 1.01 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Canine CD20 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD20 Antibody, hFc Tag with an affinity constant of 1.01 nM as determined in SPR assay (Biacore S200). Biotinylated Canine CD20 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD20 Antibody, hFc Tag with an affinity constant of 1.01 nM as determined in SPR assay (Biacore S200).
Immobilized Biotinylated Canine CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. Immobilized Biotinylated Canine CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
Other Available Proteins:
Finding available proteins...
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