• Express System: HEK293
  • Product Tag: C-mFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Canine PD-L1, mFc Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Canine PD-1, hFc Tag (Cat. PD1-DM201) with the EC50 of 53.3ng/ml determined by ELISA.
  • Amino Acid Range: Phe19-Arg236
Catalog #PDL-CM610B
Price
Regular price $57800
Regular price Sale price $57800
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Canine PD-L1/B7-H1 Protein (PDL-CM610B) Summary

Product Description

 Recombinant Biotinylated Canine PD-L1/B7-H1 Protein is expressed from HEK293 with mFc tag and Avi tag at the C-terminus.It contains Phe19-Arg236.

Product Background

 B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Biotinylated Canine PD-L1/B7-H1 Protein (PDL-CM610B) Specifications/Details

Protein
 PD-L1/B7-H1
Synonyms
 CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1;B7H
Accession
Species
 Canine
Biotinylated
 yes
Amino Acid Range
 Phe19-Arg236
Molecular Weight
 The protein has a predicted MW of 52.91 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Canine PD-L1, mFc Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Canine PD-1, hFc Tag (Cat. PD1-DM201) with the EC50 of 53.3ng/ml determined by ELISA.
Product Tag
 C-mFc-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Canine PD-L1, mFc Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Canine PD-1, hFc Tag (Cat. PD1-DM201) with the EC50 of 53.3ng/ml determined by ELISA. Immobilized Biotinylated Canine PD-L1, mFc Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Canine PD-1, hFc Tag (Cat. PD1-DM201) with the EC50 of 53.3ng/ml determined by ELISA.
Biotinylated Canine PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Canine PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Canine PD-L1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Canine PD-L1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...