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Biotinylated Canine VEGF164 Protein (VEG-DM464B) Summary
Product Description |
Recombinant Biotinylated Canine VEGF164 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Ala27-Arg190. |
Product Background |
Vascular endothelial growth factor (VEGF) is an essential regulator of vascularization. It is expressed as several splice variants; the major forms contain 120 amino acids, 164 amino acids, and 188 amino acids. VEGF-A164 induces an extremely heterogeneous response that includes vascular hyperpermeability, extravascular fibrin deposition, edema, angiogenesis (mother vessels, GB, daughter capillaries), arteriovenous malformations, arteriogenesis, fibrogenesis, and lymphangiogenesis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Canine VEGF164 Protein (VEG-DM464B) Specifications/Details
Protein |
VEGF164 |
Synonyms |
VEGF; VEGFA; VEGF-A; VPF |
Accession |
|
Species |
Canine |
Biotinylated |
yes |
Amino Acid Range |
Ala27-Arg190 |
Molecular Weight |
The protein has a predicted MW of 22.05 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 1.1ng/ml determined by ELISA. |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 1.1ng/ml determined by ELISA. |
|
|
Biotinylated Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Canine VEGF164 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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