Biotinylated Canine VEGF164 Protein (VEG-DM464B)

Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.

Biotinylated Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Canine VEGF164 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Amino Acid Range: Ala27-Arg190

Catalog #VEG-DM464B

Price

$578⁰⁰

Size

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Biotinylated Canine VEGF164 Protein (VEG-DM464B) Summary

Product Description

Recombinant Biotinylated Canine VEGF164 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Ala27-Arg190.

Product Background

Vascular endothelial growth factor (VEGF) is an essential regulator of vascularization. It is expressed as several splice variants; the major forms contain 120 amino acids, 164 amino acids, and 188 amino acids. VEGF-A164 induces an extremely heterogeneous response that includes vascular hyperpermeability, extravascular fibrin deposition, edema, angiogenesis (mother vessels, GB, daughter capillaries), arteriovenous malformations, arteriogenesis, fibrogenesis, and lymphangiogenesis.

Biotinylated Canine VEGF164 Protein (VEG-DM464B) Specifications/Details

Protein	VEGF164
Synonyms	VEGF; VEGFA; VEGF-A; VPF
Accession	NP_001103972.1
Species	Canine
Biotinylated	yes
Amino Acid Range	Ala27-Arg190
Molecular Weight	The protein has a predicted MW of 22.05 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Product Tag	N-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Canine VEGF164, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Bevacizumab, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
	Biotinylated Canine VEGF164 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Canine VEGF164 is greater than 90% as determined by SEC-HPLC.