Biotinylated Cynomolgus ACE2/ACEH Protein (ACE-CM401B)
Biotinylated Cynomolgus ACE2/ACEH Protein (ACE-CM401B)
Biotinylated Cynomolgus ACE2/ACEH Protein (ACE-CM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus ACE2, His Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln18-Val739
Catalog #ACE-CM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Cynomolgus ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Val739.
Product Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Product Category
Recombinant Protein
Target
ACE2/ACEH
Synonyms
ACE-2; ACEH; ACE2
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Gln18-Val739
Molecular Weight
The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
Immobilized SARS-COV-2 Spike RBD, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus ACE2, His Tag with the EC50 of 0.28ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike RBD, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus ACE2, His Tag with the EC50 of 0.28ug/ml determined by ELISA.
Biotinylated Cynomolgus ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus ACE2 is greater than 95% as determined by SEC-HPLC.

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