Name: Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-CM102B)
Brand: KactusBio
SKU: APE-CM102B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) (APE-CM102B)

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag- with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).

Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 82.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ile21-Thr503

Catalog #APE-CM102B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus Alkaline Phosphatase (Placental type) /ALPP Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains Ile21-Thr503.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery

Protein	Alkaline Phosphatase (Placental type)/ALPP
Synonyms	Alkaline phosphatase; PLAP-1; ALPP; PLAP
Accession	XP_045223825.1
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Ile21-Thr503
Molecular Weight	The protein has a predicted MW of 53.69 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 82.54 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl₂ (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus ALPP, His Tag- with an affinity constant of 82.54 nM as determined in SPR assay (Biacore T200).
	Biotinylated Cynomolgus ALPP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus ALPP is greater than 95% as determined by SEC-HPLC.