• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
  • Amino Acid Range: Ser17-Glu460
Catalog #ANG-CM403B
Price
Regular price $57800
Regular price Sale price $57800
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Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM403B) Summary

Product Description

Recombinant Biotinylated Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser17-Glu460.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Biotinylated Cynomolgus ANGPTL3/Angiopoietin-like 3 Protein (ANG-CM403B) Specifications/Details

Protein
ANGPTL3/Angiopoietin-like 3
Synonyms
ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3;ANG5;Angiopoietin 5; ANGPT-5; ANGPT 5
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Ser17-Glu460
Molecular Weight
The protein has a predicted MW of 55.19 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Product Tag
C-His-Avi
Fluorophore
Biotin-labeled
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 0.05% CHAPS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Cynomolgus ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. Immobilized Biotinylated Cynomolgus ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Biotinylated Cynomolgus ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus ANGPTL3 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus ANGPTL3 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...