Name: Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410B)
Brand: KactusBio
SKU: APR-CM410B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410B)

Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.

Biotinylated Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Biotinylated Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.55 nM as determined in BLI assay (Gator® Prime).

Loaded Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus BCMA, hFc Tag with an affinity constant of 1.87 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Amino Acid Range: Lys112-Leu250

Catalog #APR-CM410B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410B) Summary

Product Description	Recombinant Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag, Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250 trimer design.
Product Background	The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein (APR-CM410B) Specifications/Details

Protein	APRIL/TNFSF13
Synonyms	CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik
Accession	A0A2K5TJA1
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Lys112-Leu250
Molecular Weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 56-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
Product Tag	N-His-Avi-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.
	Biotinylated Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Biotinylated Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.55 nM as determined in BLI assay (Gator® Prime).
	Loaded Biotinylated Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus BCMA, hFc Tag with an affinity constant of 1.87 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.