Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412B)

  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr141-Leu285

Catalog: BAF-CM412B

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Product Description Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Thr141-Leu285.
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID A0A2K5V2X4
Species Cynomolgus
Exact Sequence Thr141-Leu285
Molecular Weight The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi,N-Flag
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
Biotinylated Cynomolgus BAFF Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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