Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412B)

  • Express System: Expi293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr141-Leu285

Catalog: BAF-CM412B

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Product Description Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with His tag and Flag tag and Avi tag at the N-terminal.It contains Thr141-Leu285.
Product Category Recombinant Protein
Target BAFF/TNFSF13B/CD257 Trimer
Synonyms BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Gene/Protein ID A0A2K5V2X4
Species Cynomolgus
Exact Sequence Thr141-Leu285
Molecular Weight The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi,N-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
The purity of Biotinylated Cynomolgus BAFF Trimer is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus BAFF Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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