Name: Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412B)
Brand: KactusBio
SKU: BAF-CM412B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (BAF-CM412B)

Biotinylated Cynomolgus BAFF (Trimer), His Tag captured on CM5 Chip via streptavidin can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.40 µM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test).

Biotinylated Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

The purity of Biotinylated Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi-Flag
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.40 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr141-Leu285

Catalog #BAF-CM412B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Product Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	BAFF/TNFSF13B/CD257
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1
Accession	A0A2K5V2X4
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Thr141-Leu285
Molecular Weight	The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.40 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi-Flag
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Cynomolgus BAFF (Trimer), His Tag captured on CM5 Chip via streptavidin can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.40 µM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test).
	Biotinylated Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Biotinylated Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.