Name: Biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1B)
Brand: KactusBio
SKU: BTN-CM4A1B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus BTN3A1, His Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln1-Trp219

Catalog #BTN-CM4A1B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln1-Trp219.
Product Background	The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Product Category	Recombinant Protein

Target	BTN3A1/CD277
Synonyms	CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Accession	A0A330KWZ1
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gln1-Trp219
Molecular Weight	The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus BTN3A1, His Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus BTN3A1, His Tag with the EC50 of 2.1ng/ml determined by ELISA.
	Biotinylated Cynomolgus BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus BTN3A1 is greater than 95% as determined by SEC-HPLC.

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